5/16/2023 0 Comments Aruna jayaraman![]() ![]() We demonstrate that overexpression and/or tumorigenic activation of the Smo proto-oncogene mediates c-myc overexpression which plays a critical role in hepatocarcinogenesis and suggests that Smo is a prognostic factor in HCC tumorigenesis. These data support our hypothesis that Hh signaling is dysregulated in human hepatocarcinogenesis. In Hep3B cells, cyclopamine, an inhibitor of wild-type SMO, had no effect, but KAAD-cyclopamine, a blocker of oncogenic SMO, inhibited Hh signaling activity by 50%, decreased expression of the hepatocarcinogenic oncogene, c-myc, by 8-fold, and inhibited the growth rate of Hep3B cells by 94%. We also demonstrated that HCC cell lines (HepG2 and Hep3B) expressed Hh pathway components and activated Hh transcriptional targets. In one tumor we identified a novel Smo mutation in an evolutionarily conserved residue. Here we show that in HCCs, overexpression of the Smo proto-oncogene, as well as an increase in the stoichiometric ratio of Smo to Ptc mRNA levels, correlated with tumor size, a prognostic indicator in HCC biology. In an unoccupied state, PTC normally functions as a tumor suppressor that inhibits Smoothened (SMO), a proto-oncoprotein, from activating downstream components and transcription of target genes. Previously, Aruna was a Senior Recruiter at Wolver ine Technical Staffing and also held positions at Barracuda, VisionIT, Kyyba, IBM. This pathway is activated when Hh ligand binds to its receptor, Patched (PTC). Aruna Jayaraman is a Manager, Recruiting at Digital Management Inc based in Bethesda, Maryland. Thus, we hypothesized that hepatocarcinogenesis may involve regulation of Hh signaling. Although normal hepatocytes lack Hh signaling, activation of the Hh pathway in endodermal progenitors is required for liver development. But I have enjoyed singing with the audience and believe in giving them the experience, however small,” says Jayashri, who was nominated at the 2013 Academy Awards for singing Pi’s Lullaby in Ang Lee’s Life of Pi (2012).Hedgehog (Hh) pathway activation promotes tumors in several endodermally derived tissues, but its role in the pathogenesis of hepatocellular carcinoma (HCC) is unknown. “If I’m delving into Bhairavi or Todi, then it’s a lonely journey. She asks her audience to sing along with her in Carnatic classical concerts, which is rare for classical musicians, who are often caught in the idea of intricacies and technicalities. ![]() She breaks the mould that classical music necessitates and has traversed genres, from Hindustani to Carnatic and film music (such as Vaseegara/Zara zara, Narumugaye and so on). Jayashri is accessible to her listeners, old and young. “On stage, flanked by my fellow musicians, we attempt to be on a journey together, to reach destinations that music is capable of taking us to,” says Jayashri, who has collaborated with a plethora of artistes including Shubha Mudgal, TM Krishna, and Alarmel Valli, among others. With her singular focus, grammar and poise, her concerts became an experience for her listeners. Successive seasons at Chennai’s famous Margazhi festival and, soon enough, Jayashri became the toast of the town. The calm she achieved then would reflect in her singing years later.Ī post shared by Bombay Jayashri Ramnath opportunities started pouring in. “He taught me to befriend the swaras (musical notes), make this world my own and swim in it,” says Jayashri, who gradually understood that music can be an inward-looking personal pursuit. Her maternal grandfather was friends with the late violin virtuoso Lalgudi Jayaraman, under whom Jayashri began training. An art form that was just an expression earlier now became her identity. Her move to Chennai in the ’80s put things in perspective. She was, however, still unsure whether music was her calling. “It allowed me to learn how to project my voice,” she says. ![]() She soon landed at Mumbai’s recording studios, singing TV-commercial jingles for brands such as Bournvita and Rexona. I started to realise that this was a blessing which I may have ignored,” says Jayashri, who won a few college competitions. “My self-esteem was defined by the fact that I could sing. She discovered that her new friends appreciated her knowledge of music. It was only after she entered college (RA Podar College), in the early ’80s, that Jayashri started enjoying being on stage. It wasn’t a waste of time for her,” she says. She knew this was her dream and that I, perhaps, will be able to achieve it. There would be days when I would not speak to her. I would just go and rattle out something. She would say, ‘sing here, sing there, this temple, that stage’. A post shared by B Prasanna was about 16 when her mother began taking her to perform everywhere - at weddings, village concerts, Navaratri pandals and Ganesh Chaturthi functions. ![]()
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